ABSTRACT
【Objective】 To investigate the environmental pollution of blood collection and supply institutions by using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and evaluate its application value. 【Methods】 Colonies of air from blood donation sites, skin puncture sites of blood donors, platelet storage boxes, platelet collection equipment, object surfaces of related experimental consumables and cuff surfaces of staff after disinfection were collected, and typical colonies after cultivation were selected for microbial identification by microbial mass spectrometry and then compared with bacteria results detected in blood components from May 2017 to May 2021. 【Results】 Aseptic growth, the number of colonies ≤4.0 CFU/ dish, and the number of colonies > 4.0 CFU/dish accounted for 21.20%, 62.20% and 16.60%, respectively. The qualified rate from high to low was platelet storage box, bacteria settling in the air of blood donation room after disinfection, platelet collection equipment, skin puncture site of blood donors after disinfection, the surface of platelet consumables and the surface of medical staff's overalls. After disinfection, the blood donors' skin puncture sites were compared with other collection sites, and the t values were 2.0371, 1.508, 2.109, 1.961 and 1.778, respectively, with no significant difference (P>0.05). Thirty cases of bacterial contamination of blood components were detected from May 2017 to May 2021, among which the detection rate of apheresis platelets was the highest, and the t values were 1.731 and 2.272, relative to the contamination frequency of erythrocytes and plasma bacteria (P>0.05), while the t value was 2.875, relative to concentrated platelets, with significant difference (P<0.05). 【Conclusion】 Bacterial contamination of blood components mostly come from air bacteria settling, blood donors' arms and skin after disinfection, and surfaces of related equipment and materials. Therefore, it is of clinical significance to conduct strict disinfection of working sites, establish disinfection monitoring methods and formulate disinfection hygiene standards in blood stations.
ABSTRACT
Objective To carry out the performance evaluation on 3 hemoglobin analyzers-URIT-12,HemoCue Hb301 and CompoLab TS.Methods Verification of inter-run precision,intra-day precision,and correlation of the 3 analyzers using samples with different concentrations.Results The lower values of inter-run precision of URIT-12,HemoCue Hb301 and CompoLab TSoft were 1.46％,1.45％ and 1.32％,respectively,and the median values were 1.43％,0.56％ and 1.49％,respectively.The lower values of intra-day precision were 1.86％,1.62％ and 1.32％ respectively for URIT-12,HemoCue Hb301 and CompoLab TSoft,with the median values of 1.45％,1.95％ and 1.78％ and the higher values of 1.86％,1.74％ and 1.81％.Based on the results of Beckman-Coulter AcT5diff automatic blood cell analyzer,linear regression equation and correlation coefficient was calculated and expressed as y=0.960 2x+3.201 3,R2=0.973 4;y=1.112 1x-6.767 9,R2 =0.990 7;y=1.031 5x+0.634 7,R2=0.980 3,respectively.Conclusion The 3 hemoglobin analyzers have better inter-run precision and intra-day precision as well as good correlation with automatic blood analyzer,and thus can be applied in physical examinations before blood donation.
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Objective To carry out regularpersonnel capacity evaluation of quality control laboratory (QC Lab) staff,combinedwith the actual situation of the QC Lab and the requirement of national capacity assessment.Method Establish an evaluation program,including assessment items,frequency,eligibility criteria,etc,to effectively implement the program.Results Two evaluations were conducted for nine staff membersin the lab from 2015 to 2016.The pass rate of the four test items and operation of the instrumentwere 100％.The ability to solve the problem was variable among the staff.Conclusion According to the evaluation results,staff classification management and training will effectively promote the improvement of personnel capacity and ensure the quality of laboratory testing.
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Objective To observe the effect of fundus fluorescein angiography(FFA)for diabetic retinopathy (DR).Methods FFA results of 85 cases(161 eyes)were retrospectively analyzed.The distribution of DR,the dis-tribution of non -perfusion (NP),the relationship between diabetic maculopathy (DM)and different DR period were studied.Results The ratios of Ⅰ,Ⅱ,Ⅲ,Ⅳ,Ⅴ,Ⅵ of DR were 14.29%,44.72%,34.78%,3.11%,3.11%.NP was found in 69 eyes in 161 DR eyes.The occurrence of NP was highest (81.26%)in nasal,there was NP difference in the distribution of the different quadrants (χ2 =13.931,P <0.01),there were NP differences at different distances from the optic disc.The occurrence of NP was highest (84.06%)in midperiphera.The incidence rates of macular degeneration in Ⅰ,Ⅱ,Ⅲ,Ⅳ,Ⅴ,Ⅵ of DR were 8.70%,31.94%,58.93%,80.00%,80.00%.There was correla-tion between DMand DR (χ2 =26.093,P <0.01).Conclusion FFA can provid information for DR severity grad-ing and DM,there were positively correlations between incidence and severity of DM and DR grade,NP was usually occurred at the nasal retina and the midpefipheral retina.